MOUSE :: GTM :: DBA/2J.C57BL/6J  & CAST/Ei.C57BL/6J

W.        Genome tagged mice, global congenic strains:  DBA/2J on C57BL/6J background and CAST/Ei on C57BL/6J background

1.         Construction of mouse strains (R. Davis)
2.         Phenotyping (R. Davis)
3.         Present status of strains (R. Davis)

 

1.         Construction of mouse strains (R. Davis)

The Lusis-lab developed two sets of congenic strains in which the congenic regions span the genome with the donor strains DBA/2J (DBA) or CAST/EiJ on a C57BL/6J (B6) background.  These strains were given the name “Genome tagged mice” or GTM.  A description of the first three generations of construction using microsatellite-assisted breeding is given in:

O.A. Iakoubova, C.L. Olsson, K.M. Dains, D.A. Ross, A. Andalibi, K. Lau, J. Choi, I. Kalcheva, M. Cunanan, J. Louie, V. Nimon, M. Machrus, L.G. Bentley, C. Beauheim, S. Silvey, J. Cavalcoli, A.J. Lusis and D.B. West, Genome-tagged mice (GTM): two sets of genome-wide congenic strains. Genomics,  74  (2001), pp. 89–104. PMID: 11374905


2.         Phenotyping (R. Davis)

The DBA GTM strains were described in R.C. Davis, E.E. Schadt, D.J. Smith, E.W. Hsieh, A.C. Cervino, A. Van Nas, M. Rosales, S. Doss, H. Meng, H. Allayee and A.J. Lusis, A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background. Genomics,  86  (2005), pp. 259–270. PMID: 16039824.

The CAST/Ei strains were described in R.C. Davis, Angela Jin, M. Rosales, Suzanne Yu, Xiaoyu Xia, Kathleen Ranola, E.E. Schadt, A.J. Lusis, A genome-wide set of congenic mouse strains derived from CAST/Ei on a C57BL/6 background, Genomics, 90 ( 2007), pp. 306-313
PMID: 17600671


3.         Present status of strains (R. Davis)

We currently have none of the GTM strains as live mice.  We have worked to transfer as many strains as possible to JAX.  However, their budget constraints and the low priority for congenic strains at the time prevented us from transferring most strains. 

The strains that are available at JAX include:

gtm_table

 

In renaming the strains at JAX, some strains were confused:

Our own budget constraints have made it impossible for us to maintain live colonies.    However, we were able to set aside almost all the lines as cryopreserved sperm.  To generate live mice it will be necessary to use the sperm for in vitro fertilization or for intra-cytoplasmic sperm insertion (ICSI).  For various reasons, I would focus on using ICSI as the preferred method.  It can be carried out at UC Davis where we have had good success with reviving the few strains that have been attempted.  Their charges are $3k per strain and the progeny would need to be genotyped and bred to homozygosity.